Technical Services
The LilaLab has expertise with the following suite of in-house instrumentation which can be utilized in cooperative research proposals.
- High Pressure Liquid Chromatography (HPLC)
- Agilent 1260 Infinity HPLC equipped with DAD and ELSD detectors
- Agilent 1200 HPLC equipped with DAD and RID detectors
- Agilent 1200 HPLC equipped with DAD and FLD detectors
- Anthocyanin analysis
- Includes identification of peaks by comparison to standards and quantification of total anthocyanins as cyanidin-3-O-glucoside equivalents based on peak area measurements
- Flavonoid and phenolic acids analyses
- Identification against available standards using DAD detector
- Proanthocyanidin analysis
- HPLC on normal phase column with fluorescence detection according to degree of polymerization. Quantification based on total peak areas
- PDF of chromatogram with peak areas, identification against common available standards, and quantitation if required
- Liquid Chromatography-Mass Spectrometers (LC-MS and LC-MSn )
- Waters Xevo G2-XS QToF Quadrupole Time-of-Flight Mass Spectrometry
- Shimadzu LCMS-IT-TOF (m/z 150 – 2500, 1 ppm error)
- Agilent QTOF G6510A (m/z 150 – 2500, 5 ppm error)
- Gas Chromatography-Mass Spectrometers
- Agilent 7890 with 5975C inert XL EI/CI MSD triple axis detector
- Including method development and identification from NIST library
- Circular Dichroism Spectrometer
- Jasco J-815 Cd Spectrometer
- Polarimeter
- Jasco P-2000 Polarimeter
- Grace Reveleris® Prep Purification System
- Both flash and preparative isolation of small molecules.
- Max pressure 1700 bar
- High-Performance Counter-Current Chromatography
- Dynamic Extractions Ltd – Midi-HPCCC with Armen high-pressure pump equipped with UV/Vis detector